Composite

Part:BBa_K843008:Design

Designed by: Alişan Kayabölen   Group: iGEM12_METU   (2012-10-02)


T4 Lysis Device under control of IPTG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1427
    Illegal NheI site found at 1450
    Illegal NheI site found at 1475
    Illegal NheI site found at 1498
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1032
    Illegal AgeI site found at 1102
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since our project has a potential to be used as a biofilter, safety is a big issue. To create a very safe part, we thought that bacteria should be alive only in our system. If any escape from system occurs, bacteria should kill themselves. Thus, we thought to make their lives depending on an easy-found medium compound, IPTG.


Source

All parts used for this device is present in parts registry.

References