Composite
Part:BBa_K843008:Design
Designed by: Alişan Kayabölen Group: iGEM12_METU (2012-10-02)
T4 Lysis Device under control of IPTG
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1427
Illegal NheI site found at 1450
Illegal NheI site found at 1475
Illegal NheI site found at 1498 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1032
Illegal AgeI site found at 1102 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since our project has a potential to be used as a biofilter, safety is a big issue. To create a very safe part, we thought that bacteria should be alive only in our system. If any escape from system occurs, bacteria should kill themselves. Thus, we thought to make their lives depending on an easy-found medium compound, IPTG.
Source
All parts used for this device is present in parts registry.